"Wh-what is this? If it isn't Mario's Glove! Ah-hah! Still damp with the sweat of the living!"

—Madame Clairvoya

Mario's Glove is one of Mario's five missing items, the other four being Mario's Hat, Mario's Letter, Mario's Star, and Mario's Shoe, all of which must be brought to Madame Clairvoya so that she can provide Luigi some clues as to where Mario is located. Mario's Glove is found in the Projection Room, where a small treasure chest containing it appears after Luigi has vacuumed up every Cinema Ghost.

Even though Mario's Glove is one of Mario's missing items, Mario is shown to be wearing both in his painting. Similarly, Mario is still shown to be wearing his cap in the painting, even though it is one of his missing items.

In the Nintendo 3DS version's Hidden Mansion, Mario's Glove instead appears in the Courtyard after vacuuming up every Ceiling Surprise and Purple Bomber.

[Edit] / Nintendo 3DS version
Main characters	Main characters	Luigi • Professor E. Gadd • King Boo • Mario • Gooigi†
Ghosts	Normal	Bat • Black Bogmire • Blue Blaze • Blue Twirler • Boo • Bowling Ghost • Ceiling Surprise • Elemental Ghosts (Fire • Water • Ice) • Flash • Flying Fish • Garbage Can Ghost • Ghost Guy • Gold Ghost • Gold Mouse • Grabbing Ghost (Cinema • Mirror • Red) • Mouse (Blue • Purple) • Mr. Bones • Purple Bomber • Purple Puncher • Shining Ghost • Spark • Speedy Spirit • Temper Terror • Waiter
	Portrait Ghosts	Biff Atlas • Bogmire • Boolossus • Chauncey • Clockwork Soldiers • Floating Whirlindas • Henry and Orville • Jarvis • King Boo • Lydia • Madame Clairvoya • Melody Pianissima • Miss Petunia • Mr. Luggs • Nana • Neville • Shivers • Sir Weston • Slim Bankshot • Spooky • Sue Pea • Uncle Grimmly • Vincent Van Gore
	Other	Polterpup†
Rooms	Professor E. Gadd's Lab	Gallery • Portrificationizer Chamber • The Lab • Training Room
	Area one	Anteroom • Balcony • Foyer • Master Bedroom • Nursery • Parlor • Study • Wardrobe Room
	Area two	Ball Room • Bathroom • Boneyard • Butler's Room • Conservatory • Dining Room • Graveyard • Hidden Room • Kitchen • Laundry Room • Mirror Room • Storage Room • Washroom
	Area three	Astral Hall • Balcony • Bathroom • Billiards Room • Bottom of the Well • Courtyard • Fortune-teller's Room • Nana's Room • Observatory • Projection Room • Rec Room • Safari Room • Tea Room • The Twins' Room • Washroom
	Area four	Armory • Artist's Studio • Breaker Room • Cellar • Ceramics Studio • Clockwork Room • Cold Storage • Guest Room • Pipe Room • Roof • Sealed Room • Secret Altar • Sitting Room • Telephone Room
Items	Items	Bill • Blue Diamond • Coin • Element Medal (Fire • Ice • Water) • Gold Bar • Gold Bone† • Gold Diamond • King Boo's Crown • Mario's Glove • Mario's Hat • Mario's Letter • Mario's Shoe • Mario's Star • Pearl • Red Diamond • Stone (Blue • Green • Red)
Objects	Regular	Bomb • Boo Ball • Cheese • Mirror
	Hazards	Fake door • Fire
	Equipment	Boo Radar • Flashlight • Game Boy Horror • Poltergust 3000 • Strobulb†
Other	Other	Ghost Portrificationizer • Ghost's heart • Luigi's Mansion • Luigi's NEW Mansion • Mario's Painting
Items marked with † are exclusive to the Nintendo 3DS version.