Antibody | Rabbit anti-SETD3 antibody | Abcam | ab174662; RRID:AB_2750852 | (1:10,000) |
Antibody | Rabbit anti-Actin antibody | Sigma-Aldrich | A2066; RRID:AB_476693 | (1:1000) |
Antibody | Horseradish peroxidase- conjugated goat anti-rabbit IgG antibody | Agrisera | AS09602; RRID:AB_1966902 | (1:20,000; 1:25,000) |
Antibody | Mouse anti-His6 tag antibody | GE Healthcare; PMID: 23705015 | 27-4710-01; RRID:AB_771435 | (1:2000) |
Antibody | Horseradish peroxidase -conjugated goat anti-mouse antibody | Sigma-Aldrich; PMID: 23705015 | A2554; RRID:AB_258008 | (1:10,000) |
Cell line (Homo sapiens) | HAP1 | Horizon Discovery | C859; RRID:CVCL_Y019 | The HAP1 cell line was bought directly from Horizon Discovery (Waterbeach, UK) and has been control quality checked by the vendor |
Cell line (Cercopithecus aethiops) | COS-7 | Cell Lines Service | 605470; RRID:CVCL_0224 | The COS-7 cell line was bought directly from CLS (Eppelheim, Germany) and has been control quality checked by the vendor |
Gene (Escherichia coli) | SAH nucleosidase | NA | NCBI: NC_000913.3 | |
Gene (Bacillus subtilis) | Adenine deaminase | NA | NCBI: NC_000964.3 | |
Gene (H. sapiens) | β-actin | NA | GenBank: NM_001101.4 | |
Gene (H. sapiens) | SETD3 | NA | NCBI: NM_032233.2 | |
Gene (Rattus norvegicus) | SETD3 | NA | NCBI: XM_002726774.2 | |
Gene (H. sapiens) | Cofilin-1 | NA | NCBI: NM_005507.2 | |
Gene (H. sapiens) | Profilin-1 | NA | NCBI: NM_005022.3 | |
Recombinant DNA reagent | pCOLD I (plasmid) | Takara Bio | 3361 | |
Recombinant DNA reagent | pESC-URA (plasmid) | Agilent Technologies | 217454 | |
Recombinant DNA reagent | pEF6/Myc-His A (plasmid) | Invitrogen | V96220 | |
Recombinant DNA reagent | pSpCas0n(BB)−2A-Puro (plasmid) | Other | | Kind gift of F. Zhang, Massachusetts Institute of Technology |
Genetic reagent (C. aethiops) | pEF6/SETD3 | This paper | | Transient overexpression of the recombinant human and rat SETD3 proteins in the COS-7 cell line |
Genetic reagent (E. coli) | pCOLD I/SETD3 | This paper | | Overexpression of the recombinant human and rat SETD3 proteins in the E. coli BL21 (DE3) |
Genetic reagent (E. coli) | pCOLD I/β-actin | PMID: 20851184 | | Kind gift of M. Tamura, Ehime University |
Genetic reagent (E. coli) | pCOLD I/SAH nucleosidase | This paper | | Overexpression of the recombinant E. coli SAH nucleosidase in the E.coli BL21 (DE3) |
Genetic reagent (E. coli) | pCOLD I/adenine deaminase | This paper | | Overexpression of the recombinantB.subtilis adenine deaminase in the E. coli BL21 (DE3) |
Genetic reagent (E. coli) | pCOLD I/Cofilin-1 | This paper | | Overexpression of the recombinant human cofilin-1 in the E. coli BL21 (DE3) |
Genetic reagent (E. coli) | pCOLD I/Profilin-1 | This paper | | Overexpression of the recombinant human profilin-1 in the E. coli BL21 (DE3) |
Genetic reagent (Saccharomyces cerevisiae) | pESC-URA/β-actin | This paper | | Overexpression of the recombinant human β-actin in the S. cerevisiae BY4742 strain |
Strain, strain background (B. subtilis) | Bacillus subtilis | Sigma-Aldrich | ATCC:6633 | |
Strain, strain background (E. coli) | E. coli BL21 (DE3) | Agilent Technologies | 200131 | |
Strain, strain background (S. cerevisiae) | BY4742 | EUROSCARF | Y10000 | |
Strain, strain background (S. cerevisiae) | BY4742/β-actin | This paper | | BY4742 strain overexpressing recombinant human β-actin |
Peptide, recombinant protein | Peptide H | Caslo Laboratory | | |
Peptide, recombinant protein | Peptide A | Caslo Laboratory | | |
Peptide, recombinant protein | Peptide H3N4 | Caslo Laboratory | | |
Chemical compound, drug | S-[methyl-3H]adenosyl-L-methionine; [3H]SAM | PerkinElmer | NET155V250UC; NET155V001MC | |
Chemical compound, drug | S-[methyl-2H]adenosyl-L-methionine; [2H]SAM | C/D/N Isotopes Inc. | D-4093 | |
Software, algorithm | ProteinLynx Global Server 2.4; PLGS 2.4 | Waters | RRID:SCR_016664 | |
Software, algorithm | Quantity One | BioRad | RRID:SCR_016622 | |
Software, algorithm | GraphPad Prism 4.0 | GraphPad Software | RRID:SCR_002798 | |
Software, algorithm | Proteome Discoverer | Thermo Fisher Scientific | RRID:SCR_014477 | |
Software, algorithm | Zeiss Zen | Zeiss | RRID:SCR_013672 | |