MTRF1

MTRF1
Identifikatori
Aliasi	MTRF1
Vanjski ID-jevi	OMIM: 604601 MGI: 2384815 HomoloGene: 20903 GeneCards: MTRF1
Lokacija gena (čovjek)
Hrom.	Hromosom 13 (čovjek)
Kraj	41,263,577 bp
Lokacija gena (miš)
Hrom.	Hromosom 14 (miš)
Kraj	79,661,027 bp
Ontologija gena
Molekularna funkcija	• translation release factor activity; • translation release factor activity, codon specific; • ribosome binding;
Ćelijska komponenta	• mitohondrija; • citoplazma;
Biološki proces	• translational termination; • regulation of translational termination; • Biosinteza bjelančevina; • mitochondrial translational termination;
	Izvori:Amigo / QuickGO
Ortolozi
	9617
	211253
	ENSG00000120662
	ENSMUSG00000022022
	O75570; Q8N6Z2
	Q8K126
	NM_004294; NM_001354073; NM_001354074; NM_001354076
	NM_145960
	NP_004285; NP_001341002; NP_001341003; NP_001341005
	NP_666072
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Mitohondrijski faktor 1 translacijskog oslobađanja, znan i kao MTRF1 je ljudski gen na hromosomu 13, sekvenca 13q14.11, između bazmih prova 41,216.369 i 41,263.577.^[5]^[6]

Protein kodiran ovim genom usmjerava završetak translacije kao odgovor na peptidnu sekvencu terminacijskih kodonia. U početku se smatralo da ima ulogu u prekidu sinteze mitohondrijskih proteina. Pretpostavljeno je da mtRF1 kod kičmenjaka prepoznaje nestandardne stop kodone AGA i AGG. Alternativno, na temelju detaljnog 3D modeliranja, predloženo je da se prepoznaju zaostali ribosomi, u kojima je tRNK još uvijek vezana za peptidni lanac, ali u kojima je ribosomsko A-mjesto prazno.

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 445 aminokiselina, a molekulska težina 52 306 Da.^[7]

10	20	30	40	50
MNRHLCVWLF	RHPSLNGYLQ	CHIQLHSHQF	RQIHLDTRLQ	VFRQNRNCIL
HLLSKNWSRR	YCHQDTKMLW	KHKALQKYME	NLSKEYQTLE	QCLQHIPVNE
ENRRSLNRRH	AELAPLAAIY	QEIQETEQAI	EELESMCKSL	NKQDEKQLQE
LALEERQTID	QKINMLYNEL	FQSLVPKEKY	DKNDVILEVT	AGRTTGGDIC
QQFTREIFDM	YQNYSCYKHW	QFELLNYTPA	DYGGLHHAAA	RISGDGVYKH
LKYEGGIHRV	QRIPEVGLSS	RMQRIHTGTM	SVIVLPQPDE	VDVKLDPKDL
RIDTFRAKGA	GGQHVNKTDS	AVRLVHIPTG	LVVECQQERS	QIKNKEIAFR
VLRARLYQQI	IEKDKRQQQS	ARKLQVGTRA	QSERIRTYNF	TQDRVSDHRI
AYEVRDIKEF	LCGGKGLDQL	IQRLLQSADE	EAIAELLDEH	LKSAK

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000120662 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000022022 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Zhang Y, Spremulli LL (novembar 1998). "Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1443 (1–2): 245–50. doi:10.1016/S0167-4781(98)00223-1. PMID 9838146.
^ Hansen LL, Jørgensen R, Justesen J (2000). "Assignment of the human mitochondrial translational release factor 1 (MTRF1) to chromosome 13q14.1→q14.3 and of the human mitochondrial ribosome recycling factor (MRRF) to chromosome 9q32→q34.1 with radiation hybrid mapping". Cytogenetics and Cell Genetics. 88 (1–2): 91–2. doi:10.1159/000015494. PMID 10773675.
^ "UniProt, O75570" (jezik: англ.). Pristupljeno 3. 8. 2021.CS1 održavanje: nepoznati jezik (link)

Dopunska literatura

Nozaki Y, Matsunaga N, Ishizawa T, et al. (2008). "HMRF1L is a human mitochondrial translation release factor involved in the decoding of the termination codons UAA and UAG". Genes Cells. 13 (5): 429–38. doi:10.1111/j.1365-2443.2008.01181.x. PMID 18429816.
Dunham A, Matthews LH, Burton J, et al. (2004). "The DNA sequence and analysis of human chromosome 13". Nature. 428 (6982): 522–8. doi:10.1038/nature02379. PMC 2665288. PMID 15057823.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Research. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nature Genetics. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proceedings of the National Academy of Sciences. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Soleimanpour-Lichaei HR, Kühl I, Gaisne M, et al. (2007). "mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG". Molecular Cell. 27 (5): 745–57. doi:10.1016/j.molcel.2007.06.031. PMC 1976341. PMID 17803939.
Young DJ, Edgar CD, Murphy J, et al. (2010). "Bioinformatic, structural, and functional analyses support release factor-like MTRF1 as a protein able to decode nonstandard stop codons beginning with adenine in vertebrate mitochondria". RNA. 16 (6): 1146–55. doi:10.1261/rna.1970310. PMC 2874167. PMID 20421313.
Huynen, MA, Martijn; Duarte I; Chrzanowska-Lightowlers ZM; Nabuurs SB. (8. 5. 2012). "Structure based hypothesis of a mitochondrial ribosome rescue mechanism". Biology Direct. 7 (1): 14. doi:10.1186/1745-6150-7-14. PMC 3418547. PMID 22569235.
Lind, C; Sund J; Åqvist J (2013). "Codon-reading specificities of mitochondrial release factors and translation termination at non-standard stop codons". Nature Communications. 4: 2940. doi:10.1038/ncomms3940. PMID 24352605.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000120662 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000022022 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9838146-5] Zhang Y, Spremulli LL (novembar 1998). "Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1443 (1–2): 245–50. doi:10.1016/S0167-4781(98)00223-1. PMID 9838146.

[pmid10773675-6] Hansen LL, Jørgensen R, Justesen J (2000). "Assignment of the human mitochondrial translational release factor 1 (MTRF1) to chromosome 13q14.1→q14.3 and of the human mitochondrial ribosome recycling factor (MRRF) to chromosome 9q32→q34.1 with radiation hybrid mapping". Cytogenetics and Cell Genetics. 88 (1–2): 91–2. doi:10.1159/000015494. PMID 10773675.

[UniProt-7] "UniProt, O75570" (jezik: англ.). Pristupljeno 3. 8. 2021.CS1 održavanje: nepoznati jezik (link)

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