BAG3, CPTAC-317 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BAG3	BIS

Sequence Length (AA)	Molecular Weight (Da)
575	61595

Protein Name
BAG family molecular chaperone regulator 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSAATHSPMM	QVASGNGDRD	PLPPGWEIKI	DPQTGWPFFV	DHNSRTTTWN
60	70	80	90	100
DPRVPSEGPK	ETPSSANGPS	REGSRLPPAR	EGHPVYPQLR	PGYIPIPVLH
110	120	130	140	150
EGAENRQVHP	FHVYPQPGMQ	RFRTEAAAAA	PQRSQSPLRG	MPETTQPDKQ
160	170	180	190	200
CGQVAAAAAA	QPPASHGPER	SQSPAASDCS	SSSSSASLPS	SGRSSLGSHQ
210	220	230	240	250
LPRGYISIPV	IHEQNVTRPA	AQPSFHQAQK	THYPAQQGEY	QTHQPVYHKI
260	270	280	290	300
QGDDWEPRPL	RAASPFRSSV	QGASSREGSP	ARSSTPLHSP	SPIRVHTVVD
310	320	330	340	350
RPQQPMTHRE	TAPVSQPENK	PESKPGPVGP	ELPPGHIPIQ	VIRKEVDSKP
360	370	380	390	400
VSQKPPPPSE	KVEVKVPPAP	VPCPPPSPGP	SAVPSSPKSV	ATEERAAPST
410	420	430	440	450
APAEATPPKP	GEAEAPPKHP	GVLKVEAILE	KVQGLEQAVD	NFEGKKTDKK
460	470	480	490	500
YLMIEEYLTK	ELLALDSVDP	EGRADVRQAR	RDGVRKVQTI	LEKLEQKAID
510	520	530	540	550
VPGQVQVYEL	QPSNLEADQP	LQAIMEMGAV	AADKGKKNAG	NAEDPHTETQ
560	570	575
QPEATAAATS	NPSSMTDTPG	NPAAP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-317 Collapse assay details

Data source: Panorama

Official Gene Symbol: BAG3
Peptide Sequence: AAPSTAPAEATPPKPGEAEAPPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 396
Peptide End: 418

CPTAC ID: CPTAC-317
Peptide Molecular Mass: 2,184.1063
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Broad Institute and FHCRC
Submitting Lab PI: Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y21 (2+)	18.5	9.8	12.9	32.9	18.6	15	37.7	21	19.8	15	25	15
y19 (2+)	24.9	13.4	13.8	27.2	17.3	20.2	36.9	21.9	24.5	15	25	15
y17 (2+)	17.6	8.4	12	28.1	18.6	15.6	33.2	20.4	19.7	15	25	15
sum	14.2	7.2	10.8	25.3	17.1	13.9	29	18.6	17.6	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-318 Collapse assay details

Data source: Panorama

Official Gene Symbol: BAG3
Peptide Sequence: ELLALDSVDPEGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 461
Peptide End: 473

CPTAC ID: CPTAC-318
Peptide Molecular Mass: 1,412.7147
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Broad Institute and FHCRC
Submitting Lab PI: Steve Carr

Publication

View Details (opens in a new window)

Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	6.3	11.6	7.5	11.4	11.3	8.8	13	16.2	11.6	15	25	15
y8 (1+)	7	5.4	7	8.2	6	9.2	10.8	8.1	11.6	15	25	15
y7 (1+)	7.1	7	14.1	12.4	8.8	16.6	14.3	11.2	21.8	15	25	15
sum	4.6	5.7	5.5	8.6	6.1	6.8	9.8	8.3	8.7	15	25	15