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- La pausa ribosómica se refiere al apilamiento de ribosomas durante la traducción de la secuencia de nucleótidos de mRNA. Estos mRNA son decodificados y convertidos a una secuencia de aminoácido durante la síntesis de proteínas por los ribosomas. Dentro del ribosoma, moléculas de ARN de transferencia reconocen codones trinucleótidos específicos en el mRNA, y añaden sus aminoácidos cognados a las cadenas de proteína nacientes. Se sabe desde la década de 1980 que diferentes mRNAs se traducen a diferentes velocidades. Se piensa que la razón principal para estas diferencias es la concentración existente de variedades más raras de tRNA que limitan la velocidad en que algunos mRNA pueden ser decodificados. (es)
- Ribosomal pause refers to the queueing or stacking of ribosomes during translation of the nucleotide sequence of mRNA transcripts. These transcripts are decoded and converted into an amino acid sequence during protein synthesis by ribosomes. Due to the pause sites of some mRNA's, there is a disturbance caused in translation. Ribosomal pausing occurs in both eukaryotes and prokaryotes. A more severe pause is known as a ribosomal stall. It's been known since the 1980s that different mRNAs are translated at different rates. The main reason for these differences was thought to be the concentration of varieties of rare tRNAs limiting the rate at which some transcripts could be decoded. However, with research techniques such as ribosome profiling, it was found that at certain sites there were higher concentrations of ribosomes than average, and these pause sites were tested with specific codons. No link was found between the occupancy of specific codons and amount of their tRNAs. Thus, the early findings about rare tRNAs causing pause sites doesn't seem plausible. Two techniques can localize the ribosomal pause site in vivo; a micrococcal nuclease protection assay and isolation of polysomal transcript. Isolation of polysomal transcripts occurs by centrifuging tissue extracts through a sucrose cushion with translation elongation inhibitors, for example cycloheximide. Ribosome pausing can be detected during preprolactin synthesis on free polysomes, when the ribosome is paused the other ribosomes are tightly stacked together. When the ribosome pauses, during translation, the fragments that started to translate before the pause took place are overrepresented. However, along with the mRNA if the ribosome pauses then specific bands will be improved in the trailing edge of the ribosome. Some of the elongation inhibitors, such as: cycloheximide (in eukaryotes) or chloramphenicol, cause the ribosomes to pause and to accumulate in the start codons. Elongation Factor P regulates the ribosomal pause at polyproline in bacteria, and when there is no EFP the density of ribosomes decreases from the polyproline motifs. If there are multiple ribosome pauses, then the EFP won't resolve it. (en)
- 核糖體停滯（Ribosomal pause, Ribosomal stall）是細胞中核糖體轉譯mRNA時發生停滯的現象，在原核生物與真核生物細胞中皆會發生。等實驗技術可用於找出mRNA中發生核糖體停滯的位點。 (zh)
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- 核糖體停滯（Ribosomal pause, Ribosomal stall）是細胞中核糖體轉譯mRNA時發生停滯的現象，在原核生物與真核生物細胞中皆會發生。等實驗技術可用於找出mRNA中發生核糖體停滯的位點。 (zh)
- La pausa ribosómica se refiere al apilamiento de ribosomas durante la traducción de la secuencia de nucleótidos de mRNA. Estos mRNA son decodificados y convertidos a una secuencia de aminoácido durante la síntesis de proteínas por los ribosomas. Dentro del ribosoma, moléculas de ARN de transferencia reconocen codones trinucleótidos específicos en el mRNA, y añaden sus aminoácidos cognados a las cadenas de proteína nacientes. (es)
- Ribosomal pause refers to the queueing or stacking of ribosomes during translation of the nucleotide sequence of mRNA transcripts. These transcripts are decoded and converted into an amino acid sequence during protein synthesis by ribosomes. Due to the pause sites of some mRNA's, there is a disturbance caused in translation. Ribosomal pausing occurs in both eukaryotes and prokaryotes. A more severe pause is known as a ribosomal stall. (en)
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- Pausa ribosómica (es)
- Ribosomal pause (en)
- 核糖體停滯 (zh)
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