The 2020 NWSL Challenge Cup was a one-off tournament during the 2020 National Women's Soccer League season to mark the league's return to action from the COVID-19 pandemic. NWSL teams were required to submit finalized rosters to participate in the 2020 NWSL Challenge Cup on June 21. Roster sizes were required to be a minimum of 22 players and maximum of 28 players (24 senior players + 4 supplemental players) and contain at least three goalkeepers. Only players listed on that final roster will be permitted to participate in the tournament. Furthermore, teams could sign a maximum of 4 players they had the rights to but were not yet under contract to short-term contracts lasting the duration of the tournament. Short-term loans from teams outside the NWSL were also permissible.