Summary: Nanofluids are of immense importance to the researchers as they have significant uses industrially due to their high heat transfer rates. This new model is employed to examine the electroosmotic flow of magnetohydrodynamic nanofluids through an asymmetric microfluidic channel. Microchannel flow is driven by the electroosmosis and peristalsis mechanisms. Nanoparticles (copper, alumina and titania) with water as a base fluid have been considered. The effects of Joule heating, mixed convection, permeability of the porous medium and dissipation of energy are considered. Besides, velocity slip conditions are also encountered at the walls. Electroosmotic phenomenon is modeled through Poisson equation and Nernst-Planck equation. Debye-Hückel approximation is considered to obtain Boltzmann distribution of electric potential across electric double layer. The emerging non-linear mathematical model is solved numerically by the built-in scheme of working software. The table is inserted for numerical values of heat transfer rate at the upper wall for three different type of water-based metallic nanofluid. To verify the accuracy of results, the comparison of solutions is also shown. Entropy generation in terms of Bejan number under different parameters is also investigated. The influence of physical factors on the characteristics of heat transfer has been pointed out. Water-based copper and titania nanofluids have relatively higher temperature and heat transfer rate when compared to the alumina-water nanofluid. Porosity decays the temperature throughout the microchannel. Moreover, temperature is strongly increased for higher values of the Joule heating parameter \(\gamma\), in presence of \(Cu\)-water nanofluid. The current analysis is related to bio-inspired electrokinetic nanofluidic micropump models and incipient nanomedicine technologies.