Qinghai spruce (Picea crassifolia) is a ecologically important species in the forest ecosystem of the Qilian Mountains region in China. Natural regeneration of this species is critical to maintaining forest ecosystem function. We here analyzed several biological indicators among naturally re-generating Qinghai spruce across several elevations in the Pailugou watershed. Specifically, seedling density, basal diameter (BD), and plant height were measured, as were soil physico-chemical parameters, at 2700 m, 3000 m, and 3300 m above sea level. Differences in the biological indicators and correlations between the indicators and soil parameters were then assessed at each elevation. The results showed that soil stoichiometry was more sensitive to changes in el-evation than seedling indicators were. Furthermore, seedling density was positively correlated with soil pH, whereas BD was positively correlated with the carbon to nitrogen ratio (C/N), the carbon to phosphorus ratio, and soil organic carbon (SOC) contents. None of the analyzed soil parameters had a significant impact on elevation-specific differences in seedling density. However, soil pH, SOC, and C/N significantly affected variations in seedling diameter at each elevation. Finally, soil pH, SOC, C/N, and the carbon to phosphorus ratio significantly affected variations in seed-ling height at each elevation. This study provides a strong theoretical basis for further under-standing of the mechanisms associated with Qinghai spruce regeneration, ulti-mately contrib-uting to rational protection and management strategies for this important natural resource.