Version 1
: Received: 17 August 2024 / Approved: 19 August 2024 / Online: 19 August 2024 (12:12:54 CEST)
How to cite:
Chhetri, A.; Paudel, M.; Gompo, T. R.; Thapaliya, S. Study on Minimum Inhibitory Concentration of Selected Antibacterials against Bacterial Isolates from Canine Pyometra. Preprints2024, 2024081339. https://doi.org/10.20944/preprints202408.1339.v1
Chhetri, A.; Paudel, M.; Gompo, T. R.; Thapaliya, S. Study on Minimum Inhibitory Concentration of Selected Antibacterials against Bacterial Isolates from Canine Pyometra. Preprints 2024, 2024081339. https://doi.org/10.20944/preprints202408.1339.v1
Chhetri, A.; Paudel, M.; Gompo, T. R.; Thapaliya, S. Study on Minimum Inhibitory Concentration of Selected Antibacterials against Bacterial Isolates from Canine Pyometra. Preprints2024, 2024081339. https://doi.org/10.20944/preprints202408.1339.v1
APA Style
Chhetri, A., Paudel, M., Gompo, T. R., & Thapaliya, S. (2024). Study on Minimum Inhibitory Concentration of Selected Antibacterials against Bacterial Isolates from Canine Pyometra. Preprints. https://doi.org/10.20944/preprints202408.1339.v1
Chicago/Turabian Style
Chhetri, A., Tulsi Ram Gompo and Sharada Thapaliya. 2024 "Study on Minimum Inhibitory Concentration of Selected Antibacterials against Bacterial Isolates from Canine Pyometra" Preprints. https://doi.org/10.20944/preprints202408.1339.v1
Abstract
Minimum Inhibitory Concentration (MIC) measures the lowest concentration of antibiotic that inhibits in-vitro growth of microorganisms. Canine pyometra is a frequently occurring reproductive disorder where reliable antibiotics along with surgical excision of uterus predicts better prognosis. Scarce literature is available to facilitate proper antibiotic selection to avoid septicemia in clinical cases. The present study was conducted to determine sensitivity and potency of selected antibacterials based on MIC values against bacterial isolates obtained from Canine Pyometra in Kathmandu district, Nepal by Agar Dilution method. Isolates obtained from 63 intra-uterine samples after ovariohysterectomy were Enterococcus spp (n=41), Klebsiella spp. (n=7), Staphylococcus spp. (n=11) and Streptococcus spp. (n=4). Enrofloxacin and Amikacin were found highly sensitive (0% resistance) with a null hypothesis of resistance to all bacterial isolates and were significantly more potent than other antibacterials tested (p<0.05; Kruskal-Wallis test) based on lower MIC ranks. Similarly, ceftriaxone was found to be least potent and was highly resistant to Enterococcus spp. (75.61%) and completely resistant to other bacterial species tested (100%). Comparative results on sensitivity and potency in the study revealed Enrofloxacin and Amikacin to be better antibacterial of choice in pyometra innervation in in-vitro setting. Further clinical trials considering other equally important variables along with MIC should be conducted to predict actual clinical efficacy of antibacterials during therapeutic management of canine pyometra in Nepal.
Copyright:
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