PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Metabolomic analysis revealed the absence of the principal antimicrobial compound of Pseudomonas donghuensis P482, 7-hydroxytropolone, under restricted nutrient conditions
Version 1
: Received: 13 June 2024 / Approved: 13 June 2024 / Online: 14 June 2024 (11:01:45 CEST)
How to cite:
Matuszewska, M.; Rajewska, M.; Staropoli, A.; Vinale, F.; Marra, R.; Lorito, M.; Puchalski, M.; Czajkowski, R.; Jafra, S. Metabolomic analysis revealed the absence of the principal antimicrobial compound of Pseudomonas donghuensis P482, 7-hydroxytropolone, under restricted nutrient conditions. Preprints2024, 2024060948. https://doi.org/10.20944/preprints202406.0948.v1
Matuszewska, M.; Rajewska, M.; Staropoli, A.; Vinale, F.; Marra, R.; Lorito, M.; Puchalski, M.; Czajkowski, R.; Jafra, S. Metabolomic analysis revealed the absence of the principal antimicrobial compound of Pseudomonas donghuensis P482, 7-hydroxytropolone, under restricted nutrient conditions. Preprints 2024, 2024060948. https://doi.org/10.20944/preprints202406.0948.v1
Matuszewska, M.; Rajewska, M.; Staropoli, A.; Vinale, F.; Marra, R.; Lorito, M.; Puchalski, M.; Czajkowski, R.; Jafra, S. Metabolomic analysis revealed the absence of the principal antimicrobial compound of Pseudomonas donghuensis P482, 7-hydroxytropolone, under restricted nutrient conditions. Preprints2024, 2024060948. https://doi.org/10.20944/preprints202406.0948.v1
APA Style
Matuszewska, M., Rajewska, M., Staropoli, A., Vinale, F., Marra, R., Lorito, M., Puchalski, M., Czajkowski, R., & Jafra, S. (2024). Metabolomic analysis revealed the absence of the principal antimicrobial compound of Pseudomonas donghuensis P482, 7-hydroxytropolone, under restricted nutrient conditions. Preprints. https://doi.org/10.20944/preprints202406.0948.v1
Chicago/Turabian Style
Matuszewska, M., Robert Czajkowski and Sylwia Jafra. 2024 "Metabolomic analysis revealed the absence of the principal antimicrobial compound of Pseudomonas donghuensis P482, 7-hydroxytropolone, under restricted nutrient conditions" Preprints. https://doi.org/10.20944/preprints202406.0948.v1
Abstract
Pseudomonas donghuensis P482 exhibits broad antibacterial and antifungal activity, primarily related to the production of 7-hydroxytropolone (7-HT). However, the restricted nutritional conditions (viz. mineral medium with single carbon source) diminished the antagonistic activity of P482. In this study, we unveil profiles of the secondary metabolites from P482 and its selected mutants, using nutrient-diluted (10% tryptone soy broth, TSB) and nutrient-restricted (M9 with either 0.4% glucose or 0.4% glycerol as a sole carbon source) media to explore differences in the quantity and quality of the secondary metabolites produced. No 7-HT was found to be synthetized by P482 under restricted nutritional conditions. However, both the wild-type and the tested mutants of P482 still did express some antagonistic activity towards the plant pathogenic bacteria tested. Based on these results, we postulate that 7-HT is not the sole antimicrobial compound produced by P. donghuensis P482 and that other antimicrobials may be involved in P482 antagonistic activity under limiting-nutrient conditions.
Keywords
antimicrobials, Pseudomonas donghuensis, metabolomics, 7-hydroxytropolone; single carbon source, secondary metabolites
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.