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. 1999 Jun;65(6):2776-80.
doi: 10.1128/AEM.65.6.2776-2780.1999.

Response of pathogenic Vibrio species to high hydrostatic pressure

D L Berlin  1 , D S Herson, D T Hicks, D G Hoover
Affiliations

Response of pathogenic Vibrio species to high hydrostatic pressure

D L Berlin et al. Appl Environ Microbiol. 1999 Jun.

Abstract

Vibrio parahaemolyticus ATCC 17802, Vibrio vulnificus ATCC 27562, Vibrio cholerae O:1 ATCC 14035, Vibrio cholerae non-O:1 ATCC 14547, Vibrio hollisae ATCC 33564, and Vibrio mimicus ATCC 33653 were treated with 200 to 300 MPa for 5 to 15 min at 25 degrees C. High hydrostatic pressure inactivated all strains of pathogenic Vibrio without triggering a viable but nonculturable (VBNC) state; however, cells already existing in a VBNC state appeared to possess greater pressure resistance.

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Figures

FIG. 1
FIG. 1
Effect of high hydrostatic pressure on Vibrio vulnificus ATCC 27562 treated in ASW at 25°C measured by the four cell enumeration methods described in the text. NG, no colony growth.
FIG. 2
FIG. 2
Induction of a VBNC state in V. vulnificus ATCC 27562 by incubation at 4°C in ASW as measured by plate counts on TSAS and TCBS, as well as DVC.
FIG. 3
FIG. 3
Pressure resistance of VBNC cultures of V. parahaemolyticus (ATCC 17802) and V. vulnificus (ATCC 27562) compared to culturable cultures in mid-log stage measured by DVC.
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