Measuring nascent macromolecular synthesis in vivo is key to understanding how cells and
tissues progress through development and respond to external cues. Here we perform in�…

Regulation of cell cycle progression is essential for cell proliferation during regeneration
following injury. After appendage amputation, the axolotl (Ambystoma mexicanum)�…

After amputation, axolotl salamanders can regenerate their limbs, but the degree to which
limb regeneration recapitulates limb development remains unclear. One limitation in�…

Background Juvenile idiopathic arthritis (JIA) is a heterogeneous disease, the signs and
symptoms of which can be summarised with use of composite disease activity measures�…

In juvenile idiopathic arthritis we have seen remarkable progress in the number of available
licensed biological and small molecule treatments in the past two decades, leading to�…

Movement-induced forces are critical to correct joint formation, but it is unclear how cells
sense and respond to these mechanical cues. To study the role of mechanical stimuli in the�…

Background Ambystoma mexicanum, the axolotl salamander, is a classic model organism
used to study vertebrate regeneration. It is assumed that axolotls regenerate most tissues�…

Mammals, including humans, are severely limited in their capability for limb regeneration,
only being able to regenerate amputated digit tips. Axolotl salamanders (Ambystoma�…

In situ hybridization is a standard procedure for visualizing mRNA transcripts in tissues. The
recent adoption of fluorescent probes and new signal amplification methods have facilitated�…

Background Methotrexate (MTX) is the gold-standard first-line disease-modifying anti-
rheumatic drug for juvenile idiopathic arthritis (JIA), despite only being either effective or�…