Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei
- PMID: 9690471
- DOI: 10.1038/28615
Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei
Abstract
Until recently, fertilization was the only way to produce viable mammalian offspring, a process implicitly involving male and female gametes. However, techniques involving fusion of embryonic or fetal somatic cells with enucleated oocytes have become steadily more successful in generating cloned young. Dolly the sheep was produced by electrofusion of sheep mammary-derived cells with enucleated sheep oocytes. Here we investigate the factors governing embryonic development by introducing nuclei from somatic cells (Sertoli, neuronal and cumulus cells) taken from adult mice into enucleated mouse oocytes. We found that some enucleated oocytes receiving Sertoli or neuronal nuclei developed in vitro and implanted following transfer, but none developed beyond 8.5 days post coitum; however, a high percentage of enucleated oocytes receiving cumulus nuclei developed in vitro. Once transferred, many of these embryos implanted and, although most were subsequently resorbed, a significant proportion (2 to 2.8%) developed to term. These experiments show that for mammals, nuclei from terminally differentiated, adult somatic cells of known phenotype introduced into enucleated oocytes are capable of supporting full development.
Comment in
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Adult cloning marches on.Nature. 1998 Jul 23;394(6691):303. doi: 10.1038/28445. Nature. 1998. PMID: 9690458 No abstract available.
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Dolly is a clone--and no longer alone.Nature. 1998 Jul 23;394(6691):315-6. doi: 10.1038/28485. Nature. 1998. PMID: 9690463 No abstract available.
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