A mutant of Pseudomonas aeruginosa that lacks c-type cytochromes has a functional cyanide-insensitive oxidase
- PMID: 8598268
- DOI: 10.1111/j.1574-6968.1996.tb07976.x
A mutant of Pseudomonas aeruginosa that lacks c-type cytochromes has a functional cyanide-insensitive oxidase
Abstract
Using transposon mutagenesis and screening for the loss of the ability to oxidise the artificial electron donor N,N,N',N'-tetramethyl-p-phenylenediamine, we have isolated a mutant of Pseudomonas aeruginosa that lacks all c-type cytochromes. This mutant is unable to grow anaerobically with nitrate as a terminal electron acceptor. Analysis of its respiratory function indicates that the mutant has lost its cytochrome c oxidase-terminated respiratory pathway but the cyanide-insensitive oxidase-terminated branch remains functional. Complementation of the mutant by in vivo cloning led to recovery of the wild-type characteristics. These data are consistent with the idea that the cyanide-insensitive respiratory pathway does not contain haem c and that the pathway's terminal oxidase is a quinol oxidase.
Similar articles
-
Isolation and characterization of TMPD-oxidase mutants of Pseudomonas aeruginosa.Arch Microbiol. 1986 Apr;144(3):228-32. doi: 10.1007/BF00410952. Arch Microbiol. 1986. PMID: 3015066
-
Isolation and characterization of mutants defective in the cyanide-insensitive respiratory pathway of Pseudomonas aeruginosa.J Bacteriol. 1995 Jan;177(2):432-8. doi: 10.1128/jb.177.2.432-438.1995. J Bacteriol. 1995. PMID: 7814333 Free PMC article.
-
Membrane-bound respiratory chain of Pseudomonas aeruginosa grown aerobically. A KCN-insensitive alternate oxidase chain and its energetics.J Biochem. 1983 Apr;93(4):1137-44. doi: 10.1093/oxfordjournals.jbchem.a134239. J Biochem. 1983. PMID: 6305927
-
A cb-type cytochrome-c oxidase terminates the respiratory chain in Helicobacter pylori.Microbiology (Reading). 1996 Jul;142 ( Pt 7):1757-63. doi: 10.1099/13500872-142-7-1757. Microbiology (Reading). 1996. PMID: 8757739
-
The terminal oxidase in the marine bacterium Pseudomonas nautica 617.Biosci Rep. 1997 Jun;17(3):343-6. doi: 10.1023/a:1027392830495. Biosci Rep. 1997. PMID: 9337488 Review.
Cited by
-
The aerobic respiratory chain of Pseudomonas aeruginosa cultured in artificial urine media: Role of NQR and terminal oxidases.PLoS One. 2020 Apr 23;15(4):e0231965. doi: 10.1371/journal.pone.0231965. eCollection 2020. PLoS One. 2020. PMID: 32324772 Free PMC article.
-
Cell biology and molecular basis of denitrification.Microbiol Mol Biol Rev. 1997 Dec;61(4):533-616. doi: 10.1128/mmbr.61.4.533-616.1997. Microbiol Mol Biol Rev. 1997. PMID: 9409151 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
