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. 1986 May;166(2):541-4.
doi: 10.1128/jb.166.2.541-544.1986.

Isolation of ntrA-like mutants of Azotobacter vinelandii

E Santero, F Luque, J R Medina, M Tortolero

Isolation of ntrA-like mutants of Azotobacter vinelandii

E Santero et al. J Bacteriol. 1986 May.

Abstract

A number of chlorate-resistant mutants of Azotobacter vinelandii affected in a general control of nitrogen metabolism were isolated. These mutants could not utilize dinitrogen, nitrate, or nitrite as a nitrogen source. The reason for this inability is that they were simultaneously deficient in nitrogenase and nitrate and nitrite reductase activities. They were complemented by a cosmid carrying a DNA fragment of A. vinelandii able to complement ntrA mutants of Escherichia coli, so they seemed to be ntrA-like mutants.

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