The Inhibitory Effects of Forsythia Koreana Extracts on the Metastatic Ability of Breast Cancer Cells and Bone Resorption by Osteoclasts
- PMID: 27390737
- PMCID: PMC4933432
- DOI: 10.15430/JCP.2016.21.2.88
The Inhibitory Effects of Forsythia Koreana Extracts on the Metastatic Ability of Breast Cancer Cells and Bone Resorption by Osteoclasts
Abstract
Background: Breast cancer is the most common malignant disease in women. The patients with advanced breast cancer develop metastasis to bone. Bone metastasis and skeletal-related events by breast cancer are frequently associated with the invasiveness of breast cancer cells and osteoclasts-mediated bone resorption. Forsythia koreana is used in oriental traditional medicine to treat asthma, atopy, and allergic diseases. The aim of this study was to evaluate the inhibitory effects of F. koreana extracts on the invasion of breast cancer cells and bone resorption by osteoclasts.
Methods: Cell viability was measured by an MTT assay and the migration and invasion of MDA-MB-231 cells were detected by a Boyden chamber assay. The formation of osteoclasts and pit was detected using tartrate-resistant acid phosphatase staining and calcium phosphate-coated plates, respectively. The activities of matrix metalloproteinases (MMPs) and cathepsin K were evaluated by gelatin zymography and a cathepsin K detection kit.
Results: The fruit and leaf extracts of F. koreana significantly inhibited the invasion of MDA-MB-231 cells at noncytotoxic concentrations. The fruit extract of F. koreana reduced the transforming growth factor β1-induced migration, invasion and MMPs activities of MDA-MB-231 cells. In addition, the fruit, branch, and leaf extracts of F. koreana also inhibited the receptor activator of nuclear factor kappa-B ligand-induced osteoclast formation and osteoclast-mediated bone-resorbing activity by reducing the activities of MMPs and cathepsin K.
Conclusions: The extracts of F. koreana may possess the potential to inhibit the breast cancer-induced bone destruction through blocking invasion of breast cancer cells, osteoclastogenesis, and the activity of mature osteoclasts.
Keywords: Bone metastasis; Bone resorption; Breast neoplasm; Forsythia koreana; Osteoclast.
Figures
Similar articles
-
Artemisinin-Daumone Hybrid Inhibits Cancer Cell-Mediated Osteolysis by Targeting Cancer Cells and Osteoclasts.Cell Physiol Biochem. 2018;49(4):1460-1475. doi: 10.1159/000493449. Epub 2018 Sep 11. Cell Physiol Biochem. 2018. PMID: 30205376
-
15-deoxy-δ12,14-prostaglandin j2 inhibits osteolytic breast cancer bone metastasis and estrogen deficiency-induced bone loss.PLoS One. 2015 Apr 10;10(4):e0122764. doi: 10.1371/journal.pone.0122764. eCollection 2015. PLoS One. 2015. PMID: 25859665 Free PMC article.
-
Platycodin D Blocks Breast Cancer-Induced Bone Destruction by Inhibiting Osteoclastogenesis and the Growth of Breast Cancer Cells.Cell Physiol Biochem. 2015;36(5):1809-20. doi: 10.1159/000430152. Cell Physiol Biochem. 2015. PMID: 26184636
-
The inhibitory effect of roasted licorice extract on human metastatic breast cancer cell-induced bone destruction.Phytother Res. 2013 Dec;27(12):1776-83. doi: 10.1002/ptr.4930. Epub 2013 Feb 11. Phytother Res. 2013. PMID: 23401151
-
Matrix metalloproteinases (MMP) and cathepsin K contribute differently to osteoclastic activities.Microsc Res Tech. 2003 Aug 15;61(6):504-13. doi: 10.1002/jemt.10374. Microsc Res Tech. 2003. PMID: 12879418 Review.
Cited by
-
Cancer and apoptosis: The apoptotic activity of plant and marine natural products and their potential as targeted cancer therapeutics.Front Pharmacol. 2022 Aug 10;13:842376. doi: 10.3389/fphar.2022.842376. eCollection 2022. Front Pharmacol. 2022. PMID: 36034846 Free PMC article. Review.
-
Breast Cancer: A Global Concern, Diagnostic and Therapeutic Perspectives, Mechanistic Targets in Drug Development.Adv Pharm Bull. 2021 Sep;11(4):580-594. doi: 10.34172/apb.2021.068. Epub 2020 Oct 14. Adv Pharm Bull. 2021. PMID: 34888205 Free PMC article. Review.
-
Anticancer Effect of the Ethyl Acetate Fraction from Orostachys japonicus on MDA-MB-231 Human Breast Cancer Cells through Extensive Induction of Apoptosis, Cell Cycle Arrest, and Antimetastasis.Evid Based Complement Alternat Med. 2019 Nov 6;2019:8951510. doi: 10.1155/2019/8951510. eCollection 2019. Evid Based Complement Alternat Med. 2019. PMID: 31781282 Free PMC article.
-
Forsythia suspensa Protects against Bone Loss in Ovariectomized Mice.Nutrients. 2019 Aug 8;11(8):1831. doi: 10.3390/nu11081831. Nutrients. 2019. PMID: 31398803 Free PMC article.
-
Liensinine and Nuciferine, Bioactive Components of Nelumbo nucifera, Inhibit the Growth of Breast Cancer Cells and Breast Cancer-Associated Bone Loss.Evid Based Complement Alternat Med. 2017;2017:1583185. doi: 10.1155/2017/1583185. Epub 2017 Dec 3. Evid Based Complement Alternat Med. 2017. PMID: 29333179 Free PMC article.
References
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous