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. 2015 Mar 6;114(9):098102.
doi: 10.1103/PhysRevLett.114.098102. Epub 2015 Mar 2.

Three-dimensional reconstruction of the giant mimivirus particle with an x-ray free-electron laser

Tomas Ekeberg  1 , Martin Svenda  1 , Chantal Abergel  2 , Filipe R N C Maia  1   3 , Virginie Seltzer  2 , Jean-Michel Claverie  2 , Max Hantke  1 , Olof Jönsson  1 , Carl Nettelblad  1 , Gijs van der Schot  1 , Mengning Liang  4 , Daniel P DePonte  4 , Anton Barty  4 , M Marvin Seibert  1   5 , Bianca Iwan  1   6 , Inger Andersson  1 , N Duane Loh  7 , Andrew V Martin  8 , Henry Chapman  4   9 , Christoph Bostedt  5 , John D Bozek  5 , Ken R Ferguson  5 , Jacek Krzywinski  5 , Sascha W Epp  10 , Daniel Rolles  10   11 , Artem Rudenko  11 , Robert Hartmann  12 , Nils Kimmel  13   14 , Janos Hajdu  1   15
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Free article

Three-dimensional reconstruction of the giant mimivirus particle with an x-ray free-electron laser

Tomas Ekeberg et al. Phys Rev Lett. .
Free article

Abstract

We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.

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  • Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser.
    Ekeberg T, Svenda M, Seibert MM, Abergel C, Maia FR, Seltzer V, DePonte DP, Aquila A, Andreasson J, Iwan B, Jönsson O, Westphal D, Odić D, Andersson I, Barty A, Liang M, Martin AV, Gumprecht L, Fleckenstein H, Bajt S, Barthelmess M, Coppola N, Claverie JM, Loh ND, Bostedt C, Bozek JD, Krzywinski J, Messerschmidt M, Bogan MJ, Hampton CY, Sierra RG, Frank M, Shoeman RL, Lomb L, Foucar L, Epp SW, Rolles D, Rudenko A, Hartmann R, Hartmann A, Kimmel N, Holl P, Weidenspointner G, Rudek B, Erk B, Kassemeyer S, Schlichting I, Strüder L, Ullrich J, Schmidt C, Krasniqi F, Hauser G, Reich C, Soltau H, Schorb S, Hirsemann H, Wunderer C, Graafsma H, Chapman H, Hajdu J. Ekeberg T, et al. Sci Data. 2016 Aug 1;3:160060. doi: 10.1038/sdata.2016.60. Sci Data. 2016. PMID: 27479754 Free PMC article.

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