Three-dimensional reconstruction of the giant mimivirus particle with an x-ray free-electron laser
- PMID: 25793853
- DOI: 10.1103/PhysRevLett.114.098102
Three-dimensional reconstruction of the giant mimivirus particle with an x-ray free-electron laser
Abstract
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.
Comment in
-
Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser.Sci Data. 2016 Aug 1;3:160060. doi: 10.1038/sdata.2016.60. Sci Data. 2016. PMID: 27479754 Free PMC article.
Similar articles
-
Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser.Sci Data. 2016 Aug 1;3:160060. doi: 10.1038/sdata.2016.60. Sci Data. 2016. PMID: 27479754 Free PMC article.
-
Atomic structure of a single large biomolecule from diffraction patterns of random orientations.J Struct Biol. 2012 Jul;179(1):41-5. doi: 10.1016/j.jsb.2012.04.014. Epub 2012 May 2. J Struct Biol. 2012. PMID: 22575364
-
Single mimivirus particles intercepted and imaged with an X-ray laser.Nature. 2011 Feb 3;470(7332):78-81. doi: 10.1038/nature09748. Nature. 2011. PMID: 21293374 Free PMC article.
-
A minimal view of single-particle imaging with X-ray lasers.Philos Trans R Soc Lond B Biol Sci. 2014 Jul 17;369(1647):20130328. doi: 10.1098/rstb.2013.0328. Philos Trans R Soc Lond B Biol Sci. 2014. PMID: 24914155 Free PMC article. Review.
-
Exploring soft matter with x-rays: from the discovery of the DNA structure to the challenges of free electron lasers.J Phys Condens Matter. 2010 Aug 18;22(32):323102. doi: 10.1088/0953-8984/22/32/323102. Epub 2010 Jul 15. J Phys Condens Matter. 2010. PMID: 21386476 Review.
Cited by
-
Coherent X-ray diffraction imaging of single particles: background impact on 3D reconstruction.J Appl Crystallogr. 2024 Aug 30;57(Pt 5):1384-1391. doi: 10.1107/S1600576724006101. eCollection 2024 Oct 1. J Appl Crystallogr. 2024. PMID: 39387090 Free PMC article.
-
A predicted model-aided one-step classification-multireconstruction algorithm for X-ray free-electron laser single-particle imaging.IUCrJ. 2024 Sep 1;11(Pt 5):891-900. doi: 10.1107/S2052252524007851. IUCrJ. 2024. PMID: 39194258 Free PMC article.
-
Correcting angular distortions in Bragg coherent X-ray diffraction imaging.J Synchrotron Radiat. 2024 Sep 1;31(Pt 5):1308-1316. doi: 10.1107/S1600577524006507. Epub 2024 Aug 8. J Synchrotron Radiat. 2024. PMID: 39116009 Free PMC article.
-
Finding the semantic similarity in single-particle diffraction images using self-supervised contrastive projection learning.NPJ Comput Mater. 2023;9(1):24. doi: 10.1038/s41524-023-00966-0. Epub 2023 Feb 16. NPJ Comput Mater. 2023. PMID: 38666059 Free PMC article.
-
A workflow for single-particle structure determination via iterative phasing of rotational invariants in fluctuation X-ray scattering.J Appl Crystallogr. 2024 Mar 15;57(Pt 2):324-343. doi: 10.1107/S1600576724000992. eCollection 2024 Apr 1. J Appl Crystallogr. 2024. PMID: 38596737 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources