(Q51041496)

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The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny.

scientific article published on 11 November 2014

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Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny. (English)

1 reference

Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

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1 reference

Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

author name string

Simon Schiml

1

1 reference

Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

Friedrich Fauser

2

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Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

publication date

11 November 2014

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Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

The Plant Journal

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Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

80

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Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

6

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Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

1139-1150

1 reference

Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

Targeted DNA excision in Arabidopsis by a re-engineered homing endonuclease

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Gene targeting in maize by somatic ectopic recombination.

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Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

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Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

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Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana

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Targeted mutagenesis of duplicated genes in soybean with zinc-finger nucleases

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Targeted molecular trait stacking in cotton through targeted double-strand break induction

1 reference

https://api.crossref.org/works/10.1111%2FTPJ.12704

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In planta gene targeting

1 reference

https://api.crossref.org/works/10.1111%2FTPJ.12704

21 January 2018

Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.

1 reference

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Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis

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High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

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Heritable targeted mutagenesis in maize using a designed endonuclease

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Non-homologous DNA end joining in plant cells is associated with deletions and filler DNA insertions

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Isolation and biochemical analysis of ethyl methanesulfonate-induced alcohol dehydrogenase null mutants ofArabidopsis thaliana (L.) Heynh

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Efficient CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana and inheritance of modified genes in the T2 and T3 generations

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A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

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Species-specific double-strand break repair and genome evolution in plants

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DNA recombination in somatic plant cells: mechanisms and evolutionary consequences

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Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease.

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21 January 2018

High-efficiency TALEN-based gene editing produces disease-resistant rice.

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21 January 2018

Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.

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21 January 2018

Targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system

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https://api.crossref.org/works/10.1111%2FTPJ.12704

21 January 2018

Targeted mutagenesis using zinc-finger nucleases in Arabidopsis

1 reference

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21 January 2018

De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks

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CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

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A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures

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Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

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https://api.crossref.org/works/10.1111%2FTPJ.12704

21 January 2018

Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases

1 reference

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21 January 2018

Two unlinked double-strand breaks can induce reciprocal exchanges in plant genomes via homologous recombination and nonhomologous end joining

1 reference

https://api.crossref.org/works/10.1111%2FTPJ.12704

21 January 2018

ZFN-mediated gene targeting of the Arabidopsis protoporphyrinogen oxidase gene through Agrobacterium-mediated floral dip transformation.

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21 January 2018

Gene targeting in plants: 25 years later

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21 January 2018

Synthetic nucleases for genome engineering in plants: prospects for a bright future

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Homologous recombination in plant cells is enhanced by in vivo induction of double strand breaks into DNA by a site-specific endonuclease

1 reference

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Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination

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Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

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Integrative genomics viewer

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Abortive gap repair: underlying mechanism for Ds element formation

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Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cells

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Rapid and efficient gene modification in rice and Brachypodium using TALENs

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Targeted genome modification of crop plants using a CRISPR-Cas system.

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Precise genome modification in the crop species Zea mays using zinc-finger nucleases

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Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs

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High-frequency modification of plant genes using engineered zinc-finger nucleases

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Natural insertions in rice commonly form tandem duplications indicative of patch-mediated double-strand break induction and repair

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Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing.

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Precision genome engineering and agriculture: opportunities and regulatory challenges

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Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew.

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TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants.

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RNA-guided genetic silencing systems in bacteria and archaea

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High-frequency homologous recombination in plants mediated by zinc-finger nucleases.

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High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases

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Transcription activator-like effector nucleases enable efficient plant genome engineering.

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The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation

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Identifiers

10.1111/TPJ.12704

1 reference

Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

27 March 2018

http://europepmc.org/abstract/MED/25327456

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