Al-Zayani is a surname. Notable people with the surname include:
- Abdullatif bin Rashid Al Zayani, Bahraini engineer and Secretary-General of the Gulf Cooperation Council
- Afnan Al Zayani, Bahraini chef
- Khalil Al-Zayani, Saudi Arabian footballer and football coach
- Mona Al-Zayani, Bahraini educator and politician