Abstract
In recent years, researchers have paid more attention to the neurobehavioral study of the adult zebrafish. It is very helpful to use a convenient observation system for the zebrafish experiments. However, the existing commercial observation systems are very expensive and the homemade systems are not flexible for different experiments. In this paper, we provide an observation system that has uniform illumination, multi-function, better flexibility and lower cost. Firstly, we designed a lighting system that has the uniform illumination through the optical simulation and polynomial fitting. Secondly, we designed the observation system, which includes tank modules and fixed bracket, so that the system can meet the requirements of many experiments. Finally, we chose white LEDs as light source and the aluminum profiles to implement the system, which make the system cheap and lightweight. In this paper, the observation system we designed achieves good result in the adult zebrafish experiments.
T. Li and X. Wang contributed equally to this work.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Burne, T., et al.: Big ideas for small brains: what can psychiatry learn from worms, flies, bees and fish? Mol. Psychiatr. 16(1), 7 (2011)
Norton, W., et al.: Adult zebrafish as a model organism for behavioural genetics. BMC Neurosci. 11, 90 (2010)
Gerlai, R.: Zebrafish antipredatory responses: a future for translational research? Behav. Brain Res. 207, 223–231 (2010)
Champagne, D.L., et al.: Translating rodent behavioral repertoire to zebrafish (Danio rerio): relevance for stress research. Behav. Brain Res. 214, 332–342 (2010)
Fan, C.Y., et al.: Gene expression changes in developing zebrafish as potential markers for rapid developmental neurotoxicity screening. Neurotoxicol. Teratol. 32, 91–98 (2010)
Rihel, J., et al.: Zebrafish behavioral profiling links drugs to biological targets and rest/wake regulation. Science 327, 348–351 (2010)
Xi, Y., et al.: Impaired dopaminergic neuron development and locomotor function in zebrafish with loss of pink1 function. Eur. J. Neurosci. 31, 623–633 (2010)
Darrow, K.O., et al.: Characterization and development of courtship in zebrafish Danio rerio. Zebrafish 1, 40–45 (2004)
Saverino, C., et al.: The social zebrafish: behavioral responses to conspecific, heterospecific, and computer animated fish. Behav. Brain Res. 191, 77–87 (2008)
Salas, C., et al.: Neuropsychology of learning and memory in teleost fish. Zebrafish 3, 157–171 (2006)
Gomez-Laplaza, L.M., et al.: Latent learning in zebrafish (Danio rerio). Behav. Brain Res. 208, 509–515 (2010)
Cachat, J., et al.: Modeling withdrawal syndrome in zebrafish. Behav. Brain Res. 208, 371–376 (2010)
Egan, R.J., et al.: Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish. Behav. Brain Res. 205, 38–44 (2009)
Maximino, C., et al.: Scototaxis as anxiety-like behavior in fish. Nat. Protoc. 5, 209–216 (2010)
http://www.viewpoint.fr/en/home, http://www.viewpoint.fr/en/p/equipment. Accessed 21 Mar 2017
http://www.noldus.com/animal-behavior-reasearch/products/ethovision-xt. Accessed 21 Mar 2017
Cachat, J., et al.: Three-dimensional neurophenotyping of adult zebrafish behavior. Plos One 6(3), e17597 (2011)
Huang, C., Wu, B., Nevatia, R.: Robust object tracking by hierarchical association of detection responses. In: Forsyth, D., Torr, P., Zisserman, A. (eds.) ECCV 2008 Part II. LNCS, vol. 5303, pp. 788–801. Springer, Heidelberg (2008). doi:10.1007/978-3-540-88688-4_58
Xiang, Y., et al.: Learning to track: online multi-object tracking by decision making In: 15th International Conference on Computer Vision, Santiago, pp. 4705–4713. IEEE (2015)
Pérezescudero, A., et al.: idTracker: tracking individuals in a group by automatic identification of unmarked animals. Nat. Methods 11, 743–748 (2014)
Bhattacharya, P., et al.: Semiconductor optoelectronic devices. Phys. Today 47(12), 64 (1994)
Narukawa, Y.: White-light LEDS. Opt. Photonics News 15(4), 24–29 (2004)
Moreno, I.: Effects on illumination uniformity due to dilution on arrays of LEDs. In: Proceedings of SPIE, vol. 5529 (2004)
Acknowledgement
This work is supported by the National Natural Science Foundation of China (NSFC: 61327802, U1613220), Major Science and Technology Project of Tianjin (14ZCDZGX00801), the Key Program of Science Foundation of Tianjin (14JCZDJC31800).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing AG
About this paper
Cite this paper
Li, T., Wang, X., Sun, M., Zhao, X. (2017). Design and Implementation of the Three-Dimensional Observation System for Adult Zebrafish. In: Liu, M., Chen, H., Vincze, M. (eds) Computer Vision Systems. ICVS 2017. Lecture Notes in Computer Science(), vol 10528. Springer, Cham. https://doi.org/10.1007/978-3-319-68345-4_49
Download citation
DOI: https://doi.org/10.1007/978-3-319-68345-4_49
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-68344-7
Online ISBN: 978-3-319-68345-4
eBook Packages: Computer ScienceComputer Science (R0)