APEH
Acilamino-kiselinski otpuštajući enzim jest enzim koji je kod ljudi kodiran genom APEH sa hromosoma 3.[5][6]
Aminokiselinska sekvenca
[uredi | uredi izvor]Dužina polipeptidnog lanca je 732 aminokiseline, а molekulska težina 81.225 Da.[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MERQVLLSEP | EEAAALYRGL | SRQPALSAAC | LGPEVTTQYG | GQYRTVHTEW | ||||
| TQRDLERMEN | IRFCRQYLVF | HDGDSVVFAG | PAGNSVETRG | ELLSRESPSG | ||||
| TMKAVLRKAG | GTGPGEEKQF | LEVWEKNRKL | KSFNLSALEK | HGPVYEDDCF | ||||
| GCLSWSHSET | HLLYVAEKKR | PKAESFFQTK | ALDVSASDDE | IARLKKPDQA | ||||
| IKGDQFVFYE | DWGENMVSKS | IPVLCVLDVE | SGNISVLEGV | PENVSPGQAF | ||||
| WAPGDAGVVF | VGWWHEPFRL | GIRFCTNRRS | ALYYVDLIGG | KCELLSDDSL | ||||
| AVSSPRLSPD | QCRIVYLQYP | SLIPHHQCSQ | LCLYDWYTKV | TSVVVDVVPR | ||||
| QLGENFSGIY | CSLLPLGCWS | ADSQRVVFDS | AQRSRQDLFA | VDTQVGTVTS | ||||
| LTAGGSGGSW | KLLTIDQDLM | VAQFSTPSLP | PTLKVGFLPS | AGKEQSVLWV | ||||
| SLEEAEPIPD | IHWGIRVLQP | PPEQENVQYA | GLDFEAILLQ | PGSPPDKTQV | ||||
| PMVVMPHGGP | HSSFVTAWML | FPAMLCKMGF | AVLLVNYRGS | TGFGQDSILS | ||||
| LPGNVGHQDV | KDVQFAVEQV | LQEEHFDASH | VALMGGSHGG | FISCHLIGQY | ||||
| PETYRACVAR | NPVINIASML | GSTDIPDWCV | VEAGFPFSSD | CLPDLSVWAE | ||||
| MLDKSPIRYI | PQVKTPLLLM | LGQEDRRVPF | KQGMEYYRAL | KTRNVPVRLL | ||||
| LYPKSTHALS | EVEVESDSFM | NAVLWLRTHL | GS |
Funkcija
[uredi | uredi izvor]Ovaj gen kodira enzim acilpeptid-hidrolaza, koji katalizira hidrolizu terminalne acetilirane aminokiseline, prvenstveno iz malih acetiliranih peptida. Acetil aminokiselina formirana ovom hidrolazom dalje se obrađuje u acetat, a slobodnu aminokiselinu aminoacilaza. Ovaj gen se nalazi unutar iste regije hromosoma 3 (3p21) kao i gen aminoacilaze, a delecije na ovom lokusu su također povezane sa smanjenjem aktivnosti aminoacilaze. Acilpeptid-hidrolaza je homotetramerni protein od 300 kDa sa svakom podjedinicom koja se sastoji od po 732 aminokiselinska ostatka. Može imati važnu ulogu u uništavanju oksidativnnih oštećenih proteina u živim ćelijama. Delecije ovog genskog lokusa nalaze se u različitim tipovima karcinoma, uključujući karcinom pluća nemalih ćelija i karcinom bubrežnih ćelija.[6]
Reference
[uredi | uredi izvor]- ^ a b c GRCh38: Ensembl release 89: ENSG00000164062 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000032590 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Erlandsson R; Bergerheim US; Boldog F; Marcsek Z; Kunimi K; Lin BY; Ingvarsson S; Castresana JS; Lee WH (Oct 1990). "A gene near the D3F15S2 site on 3p is expressed in normal human kidney but not or only at a severely reduced level in 11 of 15 primary renal cell carcinomas (RCC)". Oncogene. 5 (8): 1207–11. PMID 2392324.
- ^ a b "Entrez Gene: APEH N-acylaminoacyl-peptide hydrolase".
- ^ "UniProt, P13798" (jezik: engleski). Pristupljeno 1. 11. 2021.
Dopunska literatura
[uredi | uredi izvor]- Sharma KK, Ortwerth BJ (1992). "Description of an acylpeptide hydrolase from lens". Exp. Eye Res. 54 (6): 1005–10. doi:10.1016/0014-4835(92)90165-O. PMID 1521574.
- Scaloni A, Jones WM, Barra D, et al. (1992). "Acylpeptide hydrolase: inhibitors and some active site residues of the human enzyme". J. Biol. Chem. 267 (6): 3811–8. doi:10.1016/S0021-9258(19)50598-1. PMID 1740429.
- Erlandsson R, Boldog F, Persson B, et al. (1991). "The gene from the short arm of chromosome 3, at D3F15S2, frequently deleted in renal cell carcinoma, encodes acylpeptide hydrolase". Oncogene. 6 (7): 1293–5. PMID 1861871.
- Jones WM, Scaloni A, Bossa F, et al. (1991). "Genetic relationship between acylpeptide hydrolase and acylase, two hydrolytic enzymes with similar binding but different catalytic specificities". Proc. Natl. Acad. Sci. U.S.A. 88 (6): 2194–8. doi:10.1073/pnas.88.6.2194. PMC 51196. PMID 2006156.
- Naylor SL, Marshall A, Hensel C, et al. (1989). "The DNF15S2 locus at 3p21 is transcribed in normal lung and small cell lung cancer". Genomics. 4 (3): 355–61. doi:10.1016/0888-7543(89)90342-X. PMID 2565880.
- Feese M, Scaloni A, Jones WM, et al. (1993). "Crystallization and preliminary X-ray studies of human erythrocyte acylpeptide hydrolase". J. Mol. Biol. 233 (3): 546–9. doi:10.1006/jmbi.1993.1531. PMID 8411161.
- Mitta M, Ohnogi H, Mizutani S, et al. (1996). "The nucleotide sequence of human acylamino acid-releasing enzyme". DNA Res. 3 (1): 31–5. doi:10.1093/dnares/3.1.31. PMID 8724851.
- Scaloni A, Ingallinella P, Andolfo A, et al. (1999). "Structural investigations on human erythrocyte acylpeptide hydrolase by mass spectrometric procedures". J. Protein Chem. 18 (3): 349–60. doi:10.1023/A:1021047730831. PMID 10395453. S2CID 35403281.
- Raphel V, Giardina T, Guevel L, et al. (1999). "Cloning, sequencing and further characterization of acylpeptide hydrolase from porcine intestinal mucosa". Biochim. Biophys. Acta. 1432 (2): 371–81. doi:10.1016/S0167-4838(99)00087-4. PMID 10407158.
- Fujino T, Watanabe K, Beppu M, et al. (2000). "Identification of oxidized protein hydrolase of human erythrocytes as acylpeptide hydrolase". Biochim. Biophys. Acta. 1478 (1): 102–12. doi:10.1016/S0167-4838(00)00004-2. PMID 10719179.
- Richards P, Lees J (2002). "Functional proteomics using microchannel plate detectors". Proteomics. 2 (3): 256–61. doi:10.1002/1615-9861(200203)2:3<256::AID-PROT256>3.0.CO;2-K. PMID 11921441.
- Perrier J, Giardina T, Durand A, Puigserver A (2002). "Specific enhancement of acylase I and acylpeptide hydrolase activities by the corresponding N-acetylated substrates in primary rat hepatocyte cultures". Biol. Cell. 94 (1): 45–54. doi:10.1016/S0248-4900(01)01177-7. PMID 12000146. S2CID 10936126.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Pope SN, Lee IR (2005). "Yeast two-hybrid identification of prostatic proteins interacting with human sex hormone-binding globulin". J. Steroid Biochem. Mol. Biol. 94 (1–3): 203–8. doi:10.1016/j.jsbmb.2005.01.007. PMID 15862967. S2CID 9746088.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Vanjski linkovi
[uredi | uredi izvor]- Lokacija ljudskog genoma APEH i stranica sa detaljima o genu APEH u UCSC Genome Browseru.