User:Langmath/sandbox/Cccp-1

Conserved coiled-coil protein 1 (cccp-1) is a gene identified in Caenorhabditis elegans that codes the protein CCCP-1 on chromosome III: 17.95. The best human ortholog of cccp-1 is CCDC186 (coiled-coil domain containing 186). CCCP-1 shares properties similar to that of golgins.

As supercoils, coiled coils are more fit to transfer mechanical pulling energy than single alpha-helices.

• 1 Molecular interactions
• 2 Associated effects at intracellular and behavioral levels
• 3 Expression and localization
• 4 Properties
• 5 Motifs
• 6 C-terminus
• 7 Loss of function elegans phenotypes
• 8 References

It is believed that CCCP-1 tethers onto the trans-golgi or recycling secretory vesicle via a RAB 2 anchor (elegans UNC-108, which recruits CCCP-1) as part of dense-core vesicle generation or maturation. CCCP-1 has predicted interaction with T22C8.3 (which has predicted interaction on nucleic acid). It is yet uncharacterized how CCCP-1 interacts with the cytoskeleton, but the neuronal secretory pathway (anterograde) is driven by kinesin, a myosin-like mechanical effector. This suggests CCCP-1 tethers UNC-108 either directly or indirectly to kinesin or other motor protein that travels the cytoskeleton.

CCCP-1 is involved in dense core granule maturation, locomotion and functions in regulation of locomotion involved in locomotory behavior; cccp-1 exhibits Rab GTPase binding activity. CCCP-1 also has a caspase recruitment domain (CARD domain) implying a role in apoptosis regulation.

CCCP-1 is expressed in the spermatheca, nervous system, and the intestine; cccp-1 is localized to the axon, the neuronal cell body and the perinuclear region of cytoplasm. More than 60% of intracellular CCDC186 localizes to the cell nucleus.

CCCP-1's transcript length is approximately 2400kb with a coding sequence just over 2200kb. There are two splice variants: 734 aa and 743 aa. CCCP-1's (the protein product) estimated mass is 85 kDa.

• hmmpanther: start 6, end 732
• pfscan: start 674, end 734
• superfamily: start 93, end 342
• CARD: start 683, end 743

It has been observed that the c-terminus of conserved coiled coil protein 1 (CC3) is necessary and sufficient to localize to and bind RAB 2. This region also has potential binding properties to lipid membranes. The pfscan and CARD motifs span this region. The n-terminus domain has not been characterized.

• mild uncoordination