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Acknowledgements
We thank S. Marillonnet and Icon Genetics, Halle, Germany for providing plasmid vectors, J. Win and S. Dong for help with figure preparation, and M. Smoker for help with the plant transformation. This work was supported by the Gatsby Charitable Foundation, the European Research Council (ERC), and the Biotechnology and Biological Sciences Research Council (BBSRC).
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V.N., J.D.G.J. and S.K. have filed a patent application based on this work.
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Nekrasov, V., Staskawicz, B., Weigel, D. et al. Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat Biotechnol 31, 691–693 (2013). https://doi.org/10.1038/nbt.2655
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DOI: https://doi.org/10.1038/nbt.2655
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